Beilstein J. Org. Chem.2019,15, 2184–2190, doi:10.3762/bjoc.15.215
synthesised in amorphadiene synthase-catalysed reactions from 8- and 12-methoxyfarnesyl diphosphates due to the highly plastic yet tightly controlled carbocationic chemistry of this sesquiterpene cyclase.
Keywords: artemisinin; amorphadiene synthase; oxygenated terpenoids; sesquiterpenoids; substrate
engineering; terpenes; Introduction
Amorphadiene synthase (ADS) from Artemisia annua is a key enzyme involved in the biosynthesis of the antimalarial sesquiterpene drug artemisinin (1) [1][2][3][4]. ADS catalyses the Mg2+-dependent conversion of farnesyl diphosphate (FDP, 2) to amorpha-4,11-diene (3) with
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Graphical Abstract
Scheme 1:
Mechanism of the ADS-catalysed conversion of FDP (2) to amorpha-4,11-diene (3), a biosynthetic prec...
Beilstein J. Org. Chem.2011,7, 1494–1498, doi:10.3762/bjoc.7.173
; P450 monooxygenase; substrateengineering; Introduction
P450 monooxygenases are a ubiquitous family of enzymes found in a wide variety of organisms in all domains of life. These enzymes catalyse oxidation reactions such as hydroxylation, epoxidation, N- and O-dealkylation and heteroatom oxidation
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Graphical Abstract
Figure 1:
Oxidation reactions with P450cam-RhFRed mutants.